文章摘要
孙正伟,马蓉,徐福霞,徐汉杰,赵卫东.卵巢癌细胞多药耐药中miR-193a-3p的表达及作用[J].中国临床保健杂志,2020,23(3):392-395.
卵巢癌细胞多药耐药中miR-193a-3p的表达及作用
The expression and role of miR-193a-3p in multidrug resistance of ovarian cancer cells
投稿时间:2020-01-19  
DOI:10.3969/J.issn.1672-6790.2020.03.026
中文关键词: 卵巢肿瘤  多药耐药相关蛋白质类  调控序列,核糖核酸  敏感性与特异性
英文关键词: Ovarian neoplasms  Multidrug resistance-associated proteins  Regulatory sequences,ribonucleic acid  Sensitivity and specificity 〖FL
基金项目:安徽省科技厅资助项目(1604f0804010)
作者单位E-mail
孙正伟 安徽省第二人民医院妇产科,合肥 230041 380184026@qq.com 
马蓉 安徽省妇幼保健院妇产科  
徐福霞 安徽省第二人民医院妇产科,合肥 230041  
徐汉杰 中国科学技术大学附属第一医院安徽省立医院妇产科  
赵卫东 中国科学技术大学附属第一医院安徽省立医院妇产科 victorzhao@163.com 
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中文摘要:
      目的 探讨在卵巢癌细胞多药耐药中miR-193a-3p的表达及其作用。方法 采用MTT法分析卵巢癌细胞系A2780、SKOV-3、HO8910和ES-2对5种化疗药物的敏感性,确定其半数抑制浓度(IC50)值;用定量实时PCR(qRT-PCR)方法检测miR-193a-3p;转染miR-193a-3p mimic于最低表达miR-193a-3p的相对多药最敏感的卵巢癌细胞系ES-2中,用qRT-PCR方法检测转染效率;用MTT方法在转染后相对多药最敏感的卵巢癌细胞系ES-2中检测其对上述化疗药物敏感性的差异。结果 在相对多药最敏感的卵巢癌细胞系ES-2中miR-193a-3p的表达最低,而在相对多药最耐药的卵巢癌细胞系SKOV-3中miR-193a-3p的表达最高(P<0.01);转染miR-193a-3p mimic于相对多药最敏感的卵巢癌细胞系ES-2中后,使其增加对(紫杉醇,卡铂,多西他赛)化疗药物的耐受性(P<0.01)。结论 卵巢癌细胞多药耐药的调控中可能有miR-193a-3p的参与。
英文摘要:
      Objective To investigate the expression and role of miR-193a-3p in multidrug resistance of ovarian cancer cells.Methods MTT method was used to analyse of ovarian cancer cell line A2780,SKOV-3,HO8910,ES-2 to sensitivity of 5 chemotherapeutic drugs,and the value of half maximal inhibitory concentration (IC50).The quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expression of miR-193a-3p in ovarian cancer cell lines ES-2.MiR-193a-3p mimic was transfected into ES-2,the most multidrug-sensitive ovarian cancer cell line with the lowest expression of miR-193a-3p,and the transfection efficiency was detected by qRT-PCR;Differences in sensitivity to chemotherapeutic drugs were detected by MTT assay in ES-2,the most multidrug-sensitive ovarian cancer cell line after transfection.Results The miR-193a-3p expression was the lowest in the ovarian cancer cell line ES-2,which was the most sensitive to multiple drugs,and the highest in SKOV-3,which was the most resistant to multiple drugs(P<0.01).The resistance of the most multidrug-sensitive ovarian cancer cell line (ES-2),to chemotherapeutic drugs(Paclitaxel,Carboplatin,Docetaxel) was increased by transfection with miR-193a-3p mimic (P<0.01).Conclusion MiR-193a-3p may be involved in the regulation of multidrug resistance in ovarian cancer cells.
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