文章摘要
施冰,冯振龙,赵永歧,朱玲玲,李俊峡.SB203580减轻高原低氧大鼠脑水肿的实验研究[J].中国临床保健杂志,2019,22(5):641-643.
SB203580减轻高原低氧大鼠脑水肿的实验研究
The experiment study on SB203580 decreases cerebral edema of rats exposed to hypobaric hypoxia
投稿时间:2019-04-17  
DOI:10.3969/J.issn.1672-6790.2019.05.018
中文关键词: 脑水肿  水通道蛋白质1  水通道蛋白质4  低氧  大鼠,Sprague-Dawley
英文关键词: Brain edema  Aquaporin 1  Aquaporin 4  Hypoxia  Rats,Sprague-Dawley 〖FL
基金项目:全军后勤重点课题资助项目(BBJ14L001)
作者单位E-mail
施冰 中国人民解放军总医院第七医学中心心内科,北京 100700 dr_shibing@bjmu.edu.cn 
冯振龙 中国人民解放军总医院第七医学中心心内科,北京 100700  
赵永歧 军事科学院军事认知与脑科学研究所  
朱玲玲 军事科学院军事认知与脑科学研究所  
李俊峡 中国人民解放军总医院第七医学中心心内科,北京 100700  
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中文摘要:
      探讨SB203580对于高原低氧大鼠脑组织水通道蛋白1(AQP1)和水通道蛋白4(AQP4)表达及脑水肿的影响。方法 SPF级雄性SD大鼠54只,随机分为常压常氧对照组(sham组)、低压低氧模型组(HH组)和低压低氧+药物干预组(SB203580组)。应用低压低氧实验舱模拟高原环境,构建高原低氧大鼠脑损伤模型。SB203580组每日腹腔注射P38MAPK抑制剂SB203580(10 mg/kg),连续注射7 d。采用HE染色观察大鼠脑组织病理变化。干湿比重法测定脑组织含水量,荧光定量PCR法检测脑组织AQP1和AQP4 mRNA的表达。结果 与对照组比较,低压低氧组大鼠脑组织含水量增加(P<0.05),脑组织AQP1和AQP4表达显著升高(P<0.01)。与低氧组比较,SB203580组大鼠脑含水量明显降低(P<0.05),脑组织中AQP1和AQP4 mRNA 表达显著降低(P<0.01),脑组织的病理损伤较小。结论 SB203580可减轻高原低氧大鼠AQP1和AQP4mRNA表达及脑水肿。其机制可能与SB203580抑制P38MAPK信号通路的激活相关。
英文摘要:
      Objective To explore the role of SB203580 on cerebral edema and the expression of Aquaporin 1(AQP1) and Aquaporin 4(AQP4) in rats exposed to hypobaric hypoxia.Methods Fifty-four SPF male Sprague-Dawley rats weighing 200-220 g were randomly divided into 3 groups:normbaricnomoxia group (sham group);high altitude hypobaric hypoxia group(HH group);hypobaric hypoxia + drug interventiongroup(SB203580 group).Rats in hypoxic groups were exposed to hypoxia at simulated altitude of 7 000 m above sea level for 7 days respectively in order to establish hypoxic encephaledema model.The cerebral injury rat model was established by hypobaric hypoxia cabin.The rats of SB203580 group were injected SB203580(10 mg/kg) for seven days.HE staining was used to examine brain tissue injury.Wet-dry ratio was measured to estimate cerebral edema.QRT-PCR was used to detect the expression of AQP1and AQP4 mRNA.Results The brain water content was decreased after SB203580 treatment compared with HH group(P<0.05).The expression of AQP1 and AQP4 mRNA in SB203580 group were decreased (P<0.01)and the brain damage alleviated compared with HH group.Conclusions SB203580 can alleviate the expression of AQP1 and AQP4 mRNA and cerebral edema in rats exposed to hypobaric hypoxia,suggesting P38MAPK signal pathway plays an important role in cerebral protection at high altitude.
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