文章摘要
贾少丹,李靖,刘伟,纪霞.Apelin基因rs2235306多态性与2型糖尿病早期肾损害的关系[J].中国临床保健杂志,2018,21(5):605-608.
Apelin基因rs2235306多态性与2型糖尿病早期肾损害的关系
Association between polymorphism of Apelin Gene rs2235306 and early renal damage in type 2 diabetes mellitus
投稿时间:2018-06-22  
DOI:10.3969/J.issn.1672-6790.2018.05.009
中文关键词: 糖尿病,2型  基因表达调控  肾功能不全  白蛋白尿
英文关键词: Diabetes mellitus,type 2  Gene expression regulation  Renal insufficiency  Albuminuria 〖FL
基金项目:山东省医药卫生科技发展计划项目(2015BJYB34)
作者单位E-mail
贾少丹 山东青岛市市立医院干部保健科,青岛 266071 jiashaodan1965@126.com 
李靖 山东青岛市市立医院干部保健科,青岛 266071  
刘伟 山东青岛市市立医院干部保健科,青岛 266071 liuwei701021@sohu.com 
纪霞 山东青岛市市立医院干部保健科,青岛 266071  
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中文摘要:
      目的 探讨Apelin 基因rs2235306多态性与2型糖尿病早期肾损害的关系。方法 选取121例2型糖尿病患者,根据尿清蛋白与肌酐的比值分为正常清蛋白尿组(NA组,58例)和微量清蛋白尿组(MA组,63例);选择健康志愿者60名为健康对照组(NC组)。应用四引物扩增受阻突变体系聚合酶链反应(ARMS-PCR)技术,测定上述三组人群Apelin 基因rs2235306 多态性,同时测定血清Apelin水平及生化指标。结果 (1)NC组三种基因型频率分别为TT 43%、TC 45%、CC 12%,等位基因频率为T 66%、C 34%;NA组相应为TT 40%、TC 45%、CC 15%,T 62%、C 38%;MA组相应为TT 33%、TC 40%、CC 27%,T 53%、C 47%。MA组CC基因型频率及C等位基因频率明显高于NC组(χ2分别为4.591、4.082,P值为0.032、0.043);NA组CC基因型频率及C等位基因频率与NC组比较,差异无统计学意义。(2)糖尿病人群TC+CC基因型组与TT基因型组比较,空腹胰岛素(FIns)、胰岛素抵抗指数(HOMA-IR)和Apelin水平明显升高(均P<0.05)。(3)多因素回归分析结果显示,Apelin与HOMA-IR和BMI正相关(t=4.078和2.071,均P <0.05)。结论 Apelin 基因rs2235306位点C等位基因携带可能与2型糖尿病早期肾损害有关。
英文摘要:
      Objective To investigate the relationship between polymorphism of Apelin gene rs2235306 and early renal damage in type 2 diabetes mellitus. Methods 121 patients with type 2 diabetes were divided into normal albuminuria group(58 cases in the NA group) and micro-albuminuria group(63 cases in the MA group) according to the urinary micro-albumin to creatinine ratio,with 60 healthy subjects included as controls(NC group).The genotypes of Apelin gene rs2235306 were analyzed by tetra-primer amplification refractory mutation system PCR (Tetra-primer ARMS PCR,T-ARMS-PCR) in 121 patients with type 2 diabetes and 60 healthy volunteers.At the same time,serum levels of Apelin and the other biochemical indexes were tested. Results (1) The frequencies of genotypes in NC group were TT 43%,TC 45%,CC 12%.the frequencies of alleles in NC group were T 66% and C 34%,the frequencies of genotypes in NA group were TT 40%,TC 45% and CC 15%,.the frequencies of alleles in NA group were T 62% and C 38%,the frequencies of genotypes in MA group were TT 33%,TC 40% and CC 27%,and the frequencies of alleles in MA group were T 53% and C 47%.The CC genotype and allele C frequency in the MA group were higher than those in the NC group(χ2=4.591,4.082; P=0.032,0.043).For the NA group and the NC group,the CC genotype and allele C frequency had no statistical significance.(2) In the group of patients with type 2 diabetes,serum FIns,HOMA-IR and Apelin were significant higher in genotypes TC+CC group compared with the genotype TT group(P<0.05).(3) Further multivariate regression analysis revealed that Apelin had positive correlation with HOMA-IR and BMI (t=4.078,2.071.P<0.05). Conclusion The allele C carriers of Apelin gene promoter rs2235306 may be correlated with early renal damage in type 2 diabetes mellitus.
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